METODI SPERIMENTALI IN VIVO PER L'IDENTIFICAZIONE DI INTERAZIONI TRA PROTEINE NELLE PIANTE

1. Historical view of protein-protein interaction (PPI) technology development in plants;
2. Yeast two-hybrid (Y2H) and split ubiquitin system (SUS);
3. Bimolecular Fluorescence Complementation (BiFC) in plants;
4. Split luciferase complementation assay (SLCA) in plants;
5. Co-immunoprecipitation (CoIP) in plants.

The course "In vivo experimental methods to detect proteins interaction" faces the biology behinds protein-protein interaction technologies used in plant physiology, together with advantages and disadvantages. The course includes some hours of lab activities in order to see the application of one of the methods.

The students need to have a background in plant molecular biology.

The exam concerns the organisation of a scientific protocol after the lab experience

Protocols currently used in labs

Main building and PlantLab